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An arrayed CRISPR library for individual, combinatorial and multiplexed gene knockout I

ABSTRACT: We have combined a machine-learning approach with other strategies to optimize the efficiency of sgRNAs for CRISPR screens and have constructed a genome-wide, sequence-verified, arrayed CRISPR library. This incorporates expression strategies to facilitate multiplexed or combinatorial screening. By conducting parallel loss-of-function screens, we compare our approach to existing sgRNA design and expression strategies.

ORGANISM(S): synthetic construct

PROVIDER: GSE97431 | GEO | 2017/07/20

SECONDARY ACCESSION(S): PRJNA381772

REPOSITORIES: GEO

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