Assaying the Effects of Splice Site Variants by Exon Trapping in a Mammalian Cell Line.
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ABSTRACT:
SUBMITTER: Tompson SW
PROVIDER: S-EPMC5528174 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
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Assaying the Effects of Splice Site Variants by Exon Trapping in a Mammalian Cell Line.
Tompson Stuart W SW Young Terri L TL
Bio-protocol 20170501 10
There are several <i>in silico</i> programs that endeavor to predict the functional impact of an individual's sequence variation at splice donor/acceptor sites, but experimental confirmation is problematic without a source of RNA from the individual that carries the variant. With the aid of an exon trapping vector, such as pSPL3, an investigator can test whether a splice site sequence change leads to altered RNA splicing, through expression of reference and variant mini-genes in mammalian cells ...[more]
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