Mass spectrometry captures structural intermediates in protein fiber self-assembly.
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ABSTRACT:
SUBMITTER: Landreh M
PROVIDER: S-EPMC5530726 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Publications
Mass spectrometry captures structural intermediates in protein fiber self-assembly.
Landreh Michael M Andersson Marlene M Marklund Erik G EG Jia Qiupin Q Meng Qing Q Johansson Jan J Robinson Carol V CV Robinson Carol V CV Rising Anna A
Chemical communications (Cambridge, England) 20170301 23
Self-assembling proteins, the basis for a broad range of biological scaffolds, are challenging to study using most structural biology approaches. Here we show that mass spectrometry (MS) in combination with MD simulations captures structural features of short-lived oligomeric intermediates in spider silk formation, providing direct insights into its complex assembly process. ...[more]
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