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Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein.

ABSTRACT: Serial femtosecond crystallography using ultrashort pulses from x-ray free electron lasers (XFELs) enables studies of the light-triggered dynamics of biomolecules. We used microcrystals of photoactive yellow protein (a bacterial blue light photoreceptor) as a model system and obtained high-resolution, time-resolved difference electron density maps of excellent quality with strong features; these allowed the determination of structures of reaction intermediates to a resolution of 1.6 angstroms. Our results open the way to the study of reversible and nonreversible biological reactions on time scales as short as femtoseconds under conditions that maximize the extent of reaction initiation throughout the crystal.

SUBMITTER: Tenboer J 

PROVIDER: S-EPMC4361027 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein.

Tenboer Jason J   Basu Shibom S   Zatsepin Nadia N   Pande Kanupriya K   Milathianaki Despina D   Frank Matthias M   Hunter Mark M   Boutet Sébastien S   Williams Garth J GJ   Koglin Jason E JE   Oberthuer Dominik D   Heymann Michael M   Kupitz Christopher C   Conrad Chelsie C   Coe Jesse J   Roy-Chowdhury Shatabdi S   Weierstall Uwe U   James Daniel D   Wang Dingjie D   Grant Thomas T   Barty Anton A   Yefanov Oleksandr O   Scales Jennifer J   Gati Cornelius C   Seuring Carolin C   Srajer Vukica V   Henning Robert R   Schwander Peter P   Fromme Raimund R   Ourmazd Abbas A   Moffat Keith K   Van Thor Jasper J JJ   Spence John C H JC   Fromme Petra P   Chapman Henry N HN   Schmidt Marius M  

Science (New York, N.Y.) 20141201 6214

Serial femtosecond crystallography using ultrashort pulses from x-ray free electron lasers (XFELs) enables studies of the light-triggered dynamics of biomolecules. We used microcrystals of photoactive yellow protein (a bacterial blue light photoreceptor) as a model system and obtained high-resolution, time-resolved difference electron density maps of excellent quality with strong features; these allowed the determination of structures of reaction intermediates to a resolution of 1.6 angstroms. O  ...[more]

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