Multidimensional chemical control of CRISPR-Cas9.
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ABSTRACT:
SUBMITTER: Maji B
PROVIDER: S-EPMC5531067 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Publications
Multidimensional chemical control of CRISPR-Cas9.
Maji Basudeb B Moore Christopher L CL Zetsche Bernd B Volz Sara E SE Zhang Feng F Shoulders Matthew D MD Choudhary Amit A
Nature chemical biology 20161031 1
Cas9-based technologies have transformed genome engineering and the interrogation of genomic functions, but methods to control such technologies across numerous dimensions-including dose, time, specificity, and mutually exclusive modulation of multiple genes-are still lacking. We conferred such multidimensional controls to diverse Cas9 systems by leveraging small-molecule-regulated protein degron domains. Application of our strategy to both Cas9-mediated genome editing and transcriptional activi ...[more]
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