Ontology highlight
ABSTRACT:
SUBMITTER: Gauto DF
PROVIDER: S-EPMC5531261 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Gauto Diego F DF Hessel Audrey A Rovó Petra P Kurauskas Vilius V Linser Rasmus R Schanda Paul P
Solid state nuclear magnetic resonance 20170414
Solid-state NMR spectroscopy can provide site-resolved information about protein dynamics over many time scales. Here we combine protein deuteration, fast magic-angle spinning (~45-60kHz) and proton detection to study dynamics of ubiquitin in microcrystals, and in particular a mutant in a region that undergoes microsecond motions in a β-turn region in the wild-type protein. We use <sup>15</sup>N R<sub>1ρ</sub> relaxation measurements as a function of the radio-frequency (RF) field strength, i.e. ...[more]