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Monitoring focal adhesion kinase phosphorylation dynamics in live cells.

ABSTRACT: Focal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase essential for a diverse set of cellular functions. Current methods for monitoring FAK activity in response to an extracellular stimulus lack spatiotemporal resolution and/or the ability to perform multiplex detection. Here we report on a novel approach to monitor the real-time kinase phosphorylation activity of FAK in live single cells by fluorescence lifetime imaging.

SUBMITTER: Damayanti NP

PROVIDER: S-EPMC5531600 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Monitoring focal adhesion kinase phosphorylation dynamics in live cells.

Damayanti Nur P NP Buno Kevin K Narayanan Nagarajan N Voytik Harbin Sherry L SL Deng Meng M Irudayaraj Joseph M K JMK

The Analyst 20170701 15

Focal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase essential for a diverse set of cellular functions. Current methods for monitoring FAK activity in response to an extracellular stimulus lack spatiotemporal resolution and/or the ability to perform multiplex detection. Here we report on a novel approach to monitor the real-time kinase phosphorylation activity of FAK in live single cells by fluorescence lifetime imaging. ...[more]

PMID: 28589989

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