?-carbonic anhydrases play a role in salicylic acid perception in Arabidopsis.

ABSTRACT: The plant hormone salicylic acid (SA) is required for defense responses. NON EXPRESSER OF PATHOGENESIS RELATED 1 (NPR1) and NON RECOGNITION OF BTH-4 (NRB4) are required for the response to SA in Arabidopsis (Arabidopsis thaliana). Here, we isolated several interactors of NRB4 using yeast two-hybrid assays. Two of these interactors, ?CA1 and ?CA2, are ?-carbonic anhydrase family proteins. Since double mutant ?ca1 ?ca2 plants did not show any obvious phenotype, we investigated other ?CAs and found that NRB4 also interacts with ?CA3 and ?CA4. Moreover, several ?CAs interacted with NPR1 in yeast, including one that interacted in a SA-dependent manner. This interaction was abolished in loss-of-function alleles of NPR1. Interactions between ?CAs and both NRB4 and NPR1 were also detected in planta, with evidence for a triple interaction, NRB4-?CA1-NPR1. The quintuple mutant ?ca1 ?ca2 ?ca3 ?ca4 ?ca6 showed partial insensitivity to SA. These findings suggest that one of the functions of carbonic anhydrases is to modulate the perception of SA in plants.

SUBMITTER: Medina-Puche L 

PROVIDER: S-EPMC5533460 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature