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Addressing preferred specimen orientation in single-particle cryo-EM through tilting.

ABSTRACT: We present a strategy for tackling preferred specimen orientation in single-particle cryogenic electron microscopy by employing tilts during data collection. We also describe a tool to quantify the resulting directional resolution using 3D Fourier shell correlation volumes. We applied these methods to determine the structures at near-atomic resolution of the influenza hemagglutinin trimer, which adopts a highly preferred specimen orientation, and of ribosomal biogenesis intermediates, which adopt moderately preferred orientations.

SUBMITTER: Tan YZ

PROVIDER: S-EPMC5533649 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Addressing preferred specimen orientation in single-particle cryo-EM through tilting.

Tan Yong Zi YZ Baldwin Philip R PR Davis Joseph H JH Williamson James R JR Potter Clinton S CS Carragher Bridget B Lyumkis Dmitry D

Nature methods 20170703 8

We present a strategy for tackling preferred specimen orientation in single-particle cryogenic electron microscopy by employing tilts during data collection. We also describe a tool to quantify the resulting directional resolution using 3D Fourier shell correlation volumes. We applied these methods to determine the structures at near-atomic resolution of the influenza hemagglutinin trimer, which adopts a highly preferred specimen orientation, and of ribosomal biogenesis intermediates, which adop ...[more]

PMID: 28671674

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