Dataset Information

Olive Mill Waste Enhances ?-Glucan Content in the Edible Mushroom Pleurotus eryngii.

ABSTRACT: Mushroom polysaccharides are edible polymers that have numerous reported biological functions; the most common effects are attributed to ?-glucans. In recent years, it became apparent that the less abundant ?-glucans also possess potent effects in various health conditions. Here we explore several Pleurotus species for their total, ? and ?-glucan content. Pleurotus eryngii was found to have the highest total glucan concentrations and the highest ?-glucans proportion. We also found that the stalks (stipe) of the fruit body contained higher glucan content then the caps (pileus). Since mushrooms respond markedly to changes in environmental and growth conditions, we developed cultivation methods aiming to increase the levels of ? and ?-glucans. Using olive mill solid waste (OMSW) from three-phase olive mills in the cultivation substrate. We were able to enrich the levels mainly of ?-glucans. Maximal total glucan concentrations were enhanced up to twice when the growth substrate contained 80% of OMSW compared to no OMSW. Taking together this study demonstrate that Pleurotus eryngii can serve as a potential rich source of glucans for nutritional and medicinal applications and that glucan content in mushroom fruiting bodies can be further enriched by applying OMSW into the cultivation substrate.

SUBMITTER: Avni S

PROVIDER: S-EPMC5536052 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Olive Mill Waste Enhances α-Glucan Content in the Edible Mushroom Pleurotus eryngii.

Avni Sharon S Ezove Nirit N Hanani Hilla H Yadid Itamar I Karpovsky Michal M Hayby Hilla H Gover Ofer O Hadar Yitzhak Y Schwartz Betty B Danay Ofer O

International journal of molecular sciences 20170718 7

Mushroom polysaccharides are edible polymers that have numerous reported biological functions; the most common effects are attributed to β-glucans. In recent years, it became apparent that the less abundant α-glucans also possess potent effects in various health conditions. Here we explore several <i>Pleurotus</i> species for their total, β and α-glucan content. <i>Pleurotus eryngii</i> was found to have the highest total glucan concentrations and the highest α-glucans proportion. We also found ...[more]

PMID: 28718825

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Project description:Aflatoxin B1 (AFB1) is the most harmful mycotoxin that occurs as natural contaminant of agricultural commodities, particularly maize. Practical solutions for detoxification of contaminated staples and reduction of agricultural wastes are scarce. We investigated the capability of the white-rot and edible fungus Plerotus eryngii (king oyster mushroom) to degrade AFB1 both in vitro and in a laboratory-scale mushroom cultivation, using a substrate similar to that routinely used in mushroom farms. In malt extract broth, degradation of AFB1 (500 ng/mL) by nine isolates of P. eryngii ranged from 81 to 99% after 10 days growth, and reached 100% for all isolates after 30 days. The growth of P. eryngii on solid medium (malt extract-agar, MEA) was significantly reduced at concentrations of AFB1 500 ng/mL or higher. However, the addition of 5% wheat straw to the culture medium increased the tolerance of P. eryngii to AFB1 and no inhibition was observed at a AFB1 content of 500 ng/mL; degradation of AFB1 in MEA supplemented with 5% wheat straw and 2.5% (w/v) maize flour was 71-94% after 30 days of growth. Further, AFB1 degradation by P. eryngii strain ITEM 13681 was tested in a laboratory-scale mushroom cultivation. The mushroom growth medium contained 25% (w/w) of maize spiked with AFB1 to the final content of 128 μg/kg. Pleurotus eryngii degraded up to 86% of the AFB1 in 28 days, with no significant reduction of either biological efficiency or mushroom yield. Neither the biomass produced on the mushroom substrate nor the mature basidiocarps contained detectable levels of AFB1 or its metabolite aflatoxicol, thus ruling out the translocation of these toxins through the fungal thallus. These findings make a contribution towards the development of a novel technology for remediation of AFB1- contaminated corn through the exploitation of the degradative capability of P. eryngii and its bioconversion into high nutritional value material intended for feed production.

Dataset Information

Olive Mill Waste Enhances ?-Glucan Content in the Edible Mushroom Pleurotus eryngii.

Publications

Olive Mill Waste Enhances α-Glucan Content in the Edible Mushroom Pleurotus eryngii.

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