Dataset Information

Crystal Structure of the Carboxy-Terminal Region of the Bacteriophage T4 Proximal Long Tail Fiber Protein Gp34.

ABSTRACT: Long tail fibers of bacteriophage T4 are formed by proteins gp34, gp35, gp36, and gp37, with gp34 located at the phage-proximal end and gp37 at the phage-distal, receptor-binding end. We have solved the structure of the carboxy-terminal region of gp34, consisting of amino acids 894-1289, by single-wavelength anomalous diffraction and extended the structure to amino acids 744-1289 using data collected from crystals containing longer gp34-fragments. The structure reveals three repeats of a mixed ?-? fibrous domain in residues 744 to 877. A triple-helical neck connects to an extended triple ?-helix domain (amino acids 900-1127) punctuated by two ?-prism domains. Next, a ?-prism domain decorated with short helices and extended ?-helices is present (residues 1146-1238), while the C-terminal end is capped with another short ?-helical region and three ?-hairpins. The structure provides insight into the stability of the fibrous gp34 protein.

SUBMITTER: Granell M

PROVIDER: S-EPMC5537660 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Crystal Structure of the Carboxy-Terminal Region of the Bacteriophage T4 Proximal Long Tail Fiber Protein Gp34.

Granell Meritxell M Namura Mikiyoshi M Alvira Sara S Kanamaru Shuji S van Raaij Mark J MJ

Viruses 20170630 7

Long tail fibers of bacteriophage T4 are formed by proteins gp34, gp35, gp36, and gp37, with gp34 located at the phage-proximal end and gp37 at the phage-distal, receptor-binding end. We have solved the structure of the carboxy-terminal region of gp34, consisting of amino acids 894-1289, by single-wavelength anomalous diffraction and extended the structure to amino acids 744-1289 using data collected from crystals containing longer gp34-fragments. The structure reveals three repeats of a mixed α ...[more]

PMID: 28665339

Dataset Information

Crystal Structure of the Carboxy-Terminal Region of the Bacteriophage T4 Proximal Long Tail Fiber Protein Gp34.

Publications

Crystal Structure of the Carboxy-Terminal Region of the Bacteriophage T4 Proximal Long Tail Fiber Protein Gp34.

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