Ontology highlight
ABSTRACT:
SUBMITTER: Xuan W
PROVIDER: S-EPMC5543777 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Xuan Weimin W Shao Sida S Schultz Peter G PG
Angewandte Chemie (International ed. in English) 20170403 18
The use of genetically encoded noncanonical amino acids (ncAAs) to construct crosslinks within or between proteins has emerged as a useful method to enhance protein stability, investigate protein-protein interactions, and improve the pharmacological properties of proteins. We report ncAAs with aryl carbamate side chains (PheK and FPheK) that can react with proximal nucleophilic residues to form intra- or intermolecular protein crosslinks. We evolved a pyrrolysyl-tRNA synthetase that incorporates ...[more]