Ontology highlight
ABSTRACT:
SUBMITTER: Li JC
PROVIDER: S-EPMC6426444 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20181115 47
The ability to add noncanonical amino acids to the genetic code may allow one to evolve proteins with new or enhanced properties using a larger set of building blocks. To this end, we have been able to select mutant proteins with enhanced thermal properties from a library of E. coli homoserine O-succinyltransferase ( metA) mutants containing randomly incorporated noncanonical amino acids. Here, we show that substitution of Phe 21 with ( p-benzoylphenyl)alanine (pBzF), increases the melting tempe ...[more]