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Identification of alternatively spliced TIMP-1 mRNA in cancer cell lines and colon cancer tissue.


ABSTRACT: TIMP-1 is a promising new candidate as a prognostic marker in colorectal and breast cancer. We now describe the discovery of two alternatively spliced variants of TIMP-1 mRNA. The two variants lacking exon 2 (del-2) and 5 (del-5), respectively, were identified in human cancer cell lines by RT-PCR. The del-2 variant was, furthermore, detected in extracts from 12 colorectal cancer tissue samples. By western blotting additional bands of lower molecular mass than full-length TIMP-1 were identified in tumor tissue, but not in plasma samples obtained from cancer patients. The two splice variants of TIMP-1 may hold important clinical information, and either alone or in combination with measurement of full-length TIMP-1 they may improve the prognostic and/or predictive value of TIMP-1 analyses.

SUBMITTER: Usher PA 

PROVIDER: S-EPMC5543896 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature

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Identification of alternatively spliced TIMP-1 mRNA in cancer cell lines and colon cancer tissue.

Usher P A PA   Sieuwerts A M AM   Bartels A A   Lademann U U   Nielsen H J HJ   Holten-Andersen L L   Foekens J A JA   Brünner N N   Offenberg H H  

Molecular oncology 20070521 2


TIMP-1 is a promising new candidate as a prognostic marker in colorectal and breast cancer. We now describe the discovery of two alternatively spliced variants of TIMP-1 mRNA. The two variants lacking exon 2 (del-2) and 5 (del-5), respectively, were identified in human cancer cell lines by RT-PCR. The del-2 variant was, furthermore, detected in extracts from 12 colorectal cancer tissue samples. By western blotting additional bands of lower molecular mass than full-length TIMP-1 were identified i  ...[more]

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