Identification of alternatively spliced TIMP-1 mRNA in cancer cell lines and colon cancer tissue.
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ABSTRACT:
SUBMITTER: Usher PA
PROVIDER: S-EPMC5543896 | biostudies-literature | 2007 Sep
REPOSITORIES: biostudies-literature
Publications
Identification of alternatively spliced TIMP-1 mRNA in cancer cell lines and colon cancer tissue.
Usher P A PA Sieuwerts A M AM Bartels A A Lademann U U Nielsen H J HJ Holten-Andersen L L Foekens J A JA Brünner N N Offenberg H H
Molecular oncology 20070521 2
TIMP-1 is a promising new candidate as a prognostic marker in colorectal and breast cancer. We now describe the discovery of two alternatively spliced variants of TIMP-1 mRNA. The two variants lacking exon 2 (del-2) and 5 (del-5), respectively, were identified in human cancer cell lines by RT-PCR. The del-2 variant was, furthermore, detected in extracts from 12 colorectal cancer tissue samples. By western blotting additional bands of lower molecular mass than full-length TIMP-1 were identified i ...[more]
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