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Development of SSR markers from transcriptomes for Orinus (Poaceae), an endemic of the Qinghai-Tibetan Plateau.

ABSTRACT: PREMISE OF THE STUDY:Transcriptomes were used to develop microsatellite markers for the plant genus Orinus (Poaceae), which comprises three species of grasses (O. thoroldii, O. kokonoricus, and O. intermedius) that are widely distributed in the Qinghai-Tibetan Plateau. METHODS AND RESULTS:Primer pairs were developed for 16 high-quality simple sequence repeats (SSRs) using transcriptomes. SSRs were amplified in 248 individuals representing the three species of Orinus; the number of alleles per locus ranged from one to seven, with an average of 2.6. The expected and observed heterozygosity per locus varied from 0.00 to 0.83 and from 0.00 to 1.00, respectively, with respective mean values of 0.32 and 0.34. CONCLUSIONS:These newly developed SSR markers will be valuable for evaluating the population genetic structure of Orinus throughout its range.

SUBMITTER: Liu YP

PROVIDER: S-EPMC5546167 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Development of SSR markers from transcriptomes for Orinus (Poaceae), an endemic of the Qinghai-Tibetan Plateau.

Liu Yu-Ping YP Su Xu X Luo Wen-Chun WC Lv Ting T Chen Ke-Long KL Harris A J AJ Shah Sayed Afzal SA

Applications in plant sciences 20170719 7

<h4>Premise of the study</h4>Transcriptomes were used to develop microsatellite markers for the plant genus Orinus (Poaceae), which comprises three species of grasses (O. thoroldii, O. kokonoricus, and O. intermedius) that are widely distributed in the Qinghai-Tibetan Plateau.<h4>Methods and results</h4>Primer pairs were developed for 16 high-quality simple sequence repeats (SSRs) using transcriptomes. SSRs were amplified in 248 individuals repres ...[more]

PMID: 28791207

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