Dataset Information

Development of microsatellite markers for Eurya acuminatissima (Theaceae).

ABSTRACT: PREMISE OF THE STUDY:Sixteen microsatellite markers were developed to study the fine-scale spatial genetic structure of Eurya acuminatissima, a dioecious tree species of Theaceae endemic to southern China. METHODS AND RESULTS:A total of 30 primer pairs were initially designed and tested on the basis of the transcriptome data of E. acuminatissima, of which 16 were successfully amplified and showed clear polymorphism. For these microsatellites, one to 17 alleles per locus were identified. The observed and expected heterozygosities ranged from 0 to 1.000 and 0 to 0.903, respectively. CONCLUSIONS:The microsatellite markers described here can be used to study genetic diversity and spatial genetic structure of E. acuminatissima. Furthermore, all loci were successfully cross-amplified in a related species, E. auriformis.

SUBMITTER: Shi X

PROVIDER: S-EPMC5546169 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Publications

Development of microsatellite markers for <i>Eurya acuminatissima</i> (Theaceae).

Shi Xianggang X Wu Haidan H Xie Hongxian H Zheng Yi Y Huang Yelin Y Yu Shixiao S

Applications in plant sciences 20170721 7

<h4>Premise of the study</h4>Sixteen microsatellite markers were developed to study the fine-scale spatial genetic structure of <i>Eurya acuminatissima</i>, a dioecious tree species of Theaceae endemic to southern China.<h4>Methods and results</h4>A total of 30 primer pairs were initially designed and tested on the basis of the transcriptome data of <i>E. acuminatissima</i>, of which 16 were successfully amplified and showed clear polymorphism. For these microsatellites, one to 17 alleles per lo ...[more]

PMID: 28791209

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Project description:BACKGROUND: Tea is one of the most popular beverages in the world. Many species in the Thea section of the Camellia genus can be processed for drinking and have been domesticated. However, few investigations have focused on the genetic consequence of domestication and geographic origin of landraces on tea plants using credible wild and planted populations of a single species. Here, C. taliensis provides us with a unique opportunity to explore these issues. RESULTS: Fourteen nuclear microsatellite loci were employed to determine the genetic diversity and domestication origin of C. taliensis, which were represented by 587 individuals from 25 wild, planted and recently domesticated populations. C. taliensis showed a moderate high level of overall genetic diversity. The greater reduction of genetic diversity and stronger genetic drift were detected in the wild group than in the recently domesticated group, indicating the loss of genetic diversity of wild populations due to overexploitation and habitat fragmentation. Instead of the endangered wild trees, recently domesticated individuals were used to compare with the planted trees for detecting the genetic consequence of domestication. A little and non-significant reduction in genetic diversity was found during domestication. The long life cycle, selection for leaf traits and gene flow between populations will delay the emergence of bottleneck in planted trees. Both phylogenetic and assignment analyses suggested that planted trees may have been domesticated from the adjacent central forest of western Yunnan and dispersed artificially to distant places. CONCLUSIONS: This study contributes to the knowledge about levels and distribution of genetic diversity of C. taliensis and provides new insights into genetic consequence of domestication and geographic origin of planted trees of this species. As an endemic tea source plant, wild, planted and recently domesticated C. taliensis trees should all be protected for their unique genetic characteristics, which are valuable for tea breeding.

Dataset Information

Development of microsatellite markers for Eurya acuminatissima (Theaceae).

Publications

Development of microsatellite markers for <i>Eurya acuminatissima</i> (Theaceae).

Similar Datasets

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