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Development of 18 microsatellite markers for Atractylodes japonica.

ABSTRACT:

Premise

Atractylodes japonica (Asteraceae) is endemic to East Asia, where its rhizomes are used in traditional medicine. To investigate the genetic diversity of this species, we developed polymorphic microsatellite markers.

Methods and results

We obtained a total of 175,825 simple sequence repeat (SSR) loci using the Illumina HiSeq 2500 system. Eighteen polymorphic SSR primer pairs were selected to determine heterozygosity levels and allele numbers in 80 individuals from four A. japonica populations. The levels of observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.133 to 0.892, respectively. Cross-amplification in the related species A. macrocephala and A. lancea was successful in 15 and 14 of the 18 markers, respectively.

Conclusions

These microsatellite markers will be useful for future studies involving A. japonica population genetics and breeding.

SUBMITTER: Jeong JT

PROVIDER: S-EPMC7249270 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Development of 18 microsatellite markers for <i>Atractylodes japonica</i>.

Jeong Jin-Tae JT Chung Hee H Ha Bo-Keun BK Gil Jinsu J Lee Jeong-Hoon JH Lee Yun-Ji YJ Kim Mi Ran MR Oh MyeongWon M Park Chun Geon CG Chang Jae Ki JK Hong Chang Pyo CP Park Sin-Gi SG Lee Yi Y

Applications in plant sciences 20200522 5

<h4>Premise</h4><i>Atractylodes japonica</i> (Asteraceae) is endemic to East Asia, where its rhizomes are used in traditional medicine. To investigate the genetic diversity of this species, we developed polymorphic microsatellite markers.<h4>Methods and results</h4>We obtained a total of 175,825 simple sequence repeat (SSR) loci using the Illumina HiSeq 2500 system. Eighteen polymorphic SSR primer pairs were selected to determine heterozygosity levels and allele numbers in 80 individuals from fo ...[more]

PMID: 32477846

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