Ontology highlight
ABSTRACT:
SUBMITTER: Jones BJ
PROVIDER: S-EPMC5546752 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Jones Bryan J BJ Bata Zsófia Z Kazlauskas Romas J RJ
ACS catalysis 20170515 6
Evolutionarily related hydroxynitrile lyases from rubber tree (<i>Hb</i>HNL) and from <i>Arabidopsis thaliana</i> (<i>At</i>HNL) follow different catalytic mechanisms with opposite enantioselectivity toward mandelonitrile. We hypothesized that the <i>Hb</i>HNL-like mechanism evolved from an enzyme with an <i>At</i>HNL-like mechanism. We created ancestor-like composite active-sites in each scaffold to elucidate how this transition may have occurred. Surprisingly, a composite active site in <i>Hb< ...[more]