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High carotenoid production by a halotolerant bacterium, Kocuria sp. strain QWT-12 and anticancer activity of its carotenoid.

ABSTRACT: Halophilic prokaryotes are extremophile microorganisms that grow optimally in media containing salts and almost appeared pigmented. Many of them contain high concentrations of carotenoids. Amongst 15 strains of halophilic prokaryotes isolated from industrial tannery wastewater in Qom, a Gram-stain-positive coccoid, aerobic, non-endospore-forming, halotolerant bacterium designated as strain QWT-12 showed high capacity in the production of carotenoids in a wide range of culture medium factors. 16S rRNA gene analysis showed that this strain belongs to the genus Kocuria. Carotenoids from this strain were extracted by methanol. MTT assay for extracted carotenoid was carried out against seven cancer cell lines belonging to breast, lung and prostate cancer with negative control of fibroblast cell line through six concentration levels to find out IC50. Based on statistical analysis of data from MTT assay, IC50 of 1, 4 and 8 mg/ml for MCF-7, (A549 and MDA-MB-468) and MDA-MB-231 respectively. Additionally, qualitative carotenoid determination was carried out using spectrophotometric method in 300-600 nm and thin layer chromatography, respectively. According to the obtained results from mass spectrophotometry, absorption spectrum of strain QWT-12 is similar to the absorption spectrum of the carotenoid neurosporene.

SUBMITTER: Rezaeeyan Z

PROVIDER: S-EPMC5547384 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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High carotenoid production by a halotolerant bacterium, Kocuria sp. strain QWT-12 and anticancer activity of its carotenoid.

Rezaeeyan Zahra Z Safarpour Atefeh A Amoozegar Mohammad Ali MA Babavalian Hamid H Tebyanian, Hamid H Shakeri Fatemeh F

EXCLI journal 20170612

Halophilic prokaryotes are extremophile microorganisms that grow optimally in media containing salts and almost appeared pigmented. Many of them contain high concentrations of carotenoids. Amongst 15 strains of halophilic prokaryotes isolated from industrial tannery wastewater in Qom, a Gram-stain-positive coccoid, aerobic, non-endospore-forming, halotolerant bacterium designated as strain QWT-12 showed high capacity in the production of carotenoids in a wide range of culture medium factors. 16S ...[more]

PMID: 28827999

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Project description:Introduction : The inevitable rise of antibiotic-resistant bacteria is a global health problem. These pathogens erode the utility of available antibiotics. Staphylococcus aureus is one of the major causes of community-acquired infections. The aim of work was to evaluate the marine actinomycetes for production of the antibacterial agent against pathogens. Methods : Halophilic actinomycetes were isolated, characterized and screened for production of antibacterial agent against pathogenic bacteria. The antibacterial compounds were extracted by solvent extraction and separated by TLC based bioautography. Antibacterial compound was further purified by flash chromatography followed by high-performance liquid chromatography (HPLC) techniques. The active fraction was characterized by spectroscopy techniques. The minimum inhibitory concentration of antibiotic was determined against pathogens. Results : A new halophilic actinomycetes strain rsk4 was isolated from marine water. It was designated as Kocuria sp. based on the physiological, biochemical and 16S rDNA sequencebased characters. It was able to produce broad-spectrum antibacterial compound and exhibited significant inhibitory activities against antibiotic-resistant S. aureus. The antibacterial compound was secreted optimally at 5% NaCl and neutral pH in the starch casein medium during stationary phase. The crude ethyl acetate extract was separated by chloroform-methanol, 24:1, v/v having Rf value 0.45. Bioassay of HPLC fractions confirms the presence of antibiotics picks at retention time: 3.24 minutes. The UV-Visible and mass spectra of the compound revealed that the active compound was different from other known antibiotics. The lowest minimum inhibitory concentration was recorded against S. aureus (30 µg/mL). Conclusion : The result suggests that a broad-spectrum antibacterial compound obtained from halophilic actinomycetes is effective against pathogenic bacteria. This compound may be a good alternative treatment against antibiotic-resistant pathogen S. aureus.

Dataset Information

High carotenoid production by a halotolerant bacterium, Kocuria sp. strain QWT-12 and anticancer activity of its carotenoid.

Publications

High carotenoid production by a halotolerant bacterium, Kocuria sp. strain QWT-12 and anticancer activity of its carotenoid.

Similar Datasets

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