Ontology highlight
ABSTRACT:
SUBMITTER: Roh SH
PROVIDER: S-EPMC5547627 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Roh Soung-Hun SH Hryc Corey F CF Jeong Hyun-Hwan HH Fei Xue X Jakana Joanita J Lorimer George H GH Chiu Wah W
Proceedings of the National Academy of Sciences of the United States of America 20170714 31
Single-particle electron cryo-microscopy (cryo-EM) is an emerging tool for resolving structures of conformationally heterogeneous particles; however, each structure is derived from an average of many particles with presumed identical conformations. We used a 3.5-Å cryo-EM reconstruction with imposed D7 symmetry to further analyze structural heterogeneity among chemically identical subunits in each GroEL oligomer. Focused classification of the 14 subunits in each oligomer revealed three dominant ...[more]