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Sialylation on O-glycans protects platelets from clearance by liver Kupffer cells.

ABSTRACT: Most platelet membrane proteins are modified by mucin-type core 1-derived glycans (O-glycans). However, the biological importance of O-glycans in platelet clearance is unclear. Here, we generated mice with a hematopoietic cell-specific loss of O-glycans (HC C1galt1^-/- ). These mice lack O-glycans on platelets and exhibit reduced peripheral platelet numbers. Platelets from HC C1galt1^-/- mice show reduced levels of ?-2,3-linked sialic acids and increased accumulation in the liver relative to wild-type platelets. The preferential accumulation of HC C1galt1^-/- platelets in the liver was reduced in mice lacking the hepatic asialoglycoprotein receptor [Ashwell-Morell receptor (AMR)]. However, we found that Kupffer cells are the primary cells phagocytosing HC C1galt1^-/- platelets in the liver. Our results demonstrate that hepatic AMR promotes preferential adherence to and phagocytosis of desialylated and/or HC C1galt1^-/- platelets by the Kupffer cell through its C-type lectin receptor CLEC4F. These findings provide insights into an essential role for core 1 O-glycosylation of platelets in their clearance in the liver.

SUBMITTER: Li Y

PROVIDER: S-EPMC5547648 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Sialylation on O-glycans protects platelets from clearance by liver Kupffer cells.

Li Yun Y Fu Jianxin J Ling Yun Y Yago Tadayuki T McDaniel J Michael JM Song Jianhua J Bai Xia X Kondo Yuji Y Qin Yannan Y Hoover Christopher C McGee Samuel S Shao Bojing B Liu Zhenghui Z Sonon Roberto R Azadi Parastoo P Marth Jamey D JD McEver Rodger P RP Ruan Changgeng C Xia Lijun L

Proceedings of the National Academy of Sciences of the United States of America 20170717 31

Most platelet membrane proteins are modified by mucin-type core 1-derived glycans (O-glycans). However, the biological importance of O-glycans in platelet clearance is unclear. Here, we generated mice with a hematopoietic cell-specific loss of O-glycans (HC C1galt1-/- ). These mice lack O-glycans on platelets and exhibit reduced peripheral platelet numbers. Platelets from HC C1galt1-/- mice show reduced levels of α-2,3-linked sialic acids and inc ...[more]

PMID: 28716912

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Project description:Patients with IgA nephropathy (IgAN) have elevated circulating levels of IgA1 with some O-glycans consisting of galactose (Gal)-deficient N-acetylgalactosamine (GalNAc) with or without N-acetylneuraminic acid (NeuAc). We have analyzed O-glycosylation heterogeneity of naturally asialo-IgA1 (Ale) myeloma protein that mimics Gal-deficient IgA1 (Gd-IgA1) of patients with IgAN, except that IgA1 O-glycans of IgAN patients are frequently sialylated. Specifically, serum IgA1 of healthy controls has more ?2,3-sialylated O-glycans (NeuAc attached to Gal) than ?2,6-sialylated O-glycans (NeuAc attached to GalNAc). As IgA1-producing cells from IgAN patients have an increased activity of ?2,6-sialyltransferase (ST6GalNAc), we hypothesize that such activity may promote premature sialylation of GalNAc and, thus, production of Gd-IgA1, as sialylation of GalNAc prevents subsequent Gal attachment. Distribution of NeuAc in IgA1 O-glycans may play an important role in the pathogenesis of IgAN. To better understand biological functions of NeuAc in IgA1, we established protocols for enzymatic sialylation leading to ?2,3- or ?2,6-sialylation of IgA1 O-glycans. Sialylation of Gal-deficient asialo-IgA1 (Ale) myeloma protein by an ST6GalNAc enzyme generated sialylated IgA1 that mimics the Gal-deficient IgA1 glycoforms in patients with IgAN, characterized by ?2,6-sialylated Gal-deficient GalNAc. In contrast, sialylation of the same myeloma protein by an ?2,3-sialyltransferase yielded IgA1 typical for healthy controls, characterized by ?2,3-sialylated Gal. The GalNAc-specific lectin from Helix aspersa (HAA) is used to measure levels of Gd-IgA1. We assessed HAA binding to IgA1 sialylated at Gal or GalNAc. As expected, ?2,6-sialylation of IgA1 markedly decreased reactivity with HAA. Notably, ?2,3-sialylation also decreased reactivity with HAA. Neuraminidase treatment recovered the original HAA reactivity in both instances. These results suggest that binding of a GalNAc-specific lectin is modulated by sialylation of GalNAc as well as Gal in the clustered IgA1 O-glycans. Thus, enzymatic sialylation offers a useful model to test the role of NeuAc in reactivities of the clustered O-glycans with lectins.

Dataset Information

Sialylation on O-glycans protects platelets from clearance by liver Kupffer cells.

Publications

Sialylation on O-glycans protects platelets from clearance by liver Kupffer cells.

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