Ontology highlight
ABSTRACT:
SUBMITTER: Gao L
PROVIDER: S-EPMC5548640 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Gao Linyi L Cox David B T DBT Yan Winston X WX Manteiga John C JC Schneider Martin W MW Yamano Takashi T Nishimasu Hiroshi H Nureki Osamu O Crosetto Nicola N Zhang Feng F
Nature biotechnology 20170605 8
The RNA-guided endonuclease Cpf1 is a promising tool for genome editing in eukaryotic cells. However, the utility of the commonly used Acidaminococcus sp. BV3L6 Cpf1 (AsCpf1) and Lachnospiraceae bacterium ND2006 Cpf1 (LbCpf1) is limited by their requirement of a TTTV protospacer adjacent motif (PAM) in the DNA substrate. To address this limitation, we performed a structure-guided mutagenesis screen to increase the targeting range of Cpf1. We engineered two AsCpf1 variants carrying the mutations ...[more]