Ontology highlight
ABSTRACT:
SUBMITTER: Girish TS
PROVIDER: S-EPMC5549631 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Girish Taverekere S TS McGinty Robert K RK Tan Song S
Journal of molecular biology 20160304 8
Set8 is the only mammalian monomethyltransferase responsible for H4K20me1, a methyl mark critical for genomic integrity of eukaryotic cells. We present here a structural model for how Set8 uses multivalent interactions to bind and methylate the nucleosome based on crystallographic and solution studies of the Set8/nucleosome complex. Our studies indicate that Set8 employs its i-SET and c-SET domains to engage nucleosomal DNA 1 to 1.5 turns from the nucleosomal dyad and in doing so, it positions t ...[more]