Ontology highlight
ABSTRACT:
SUBMITTER: Leivo J
PROVIDER: S-EPMC5550978 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Leivo Joni J Virjula Sanni S Vanhatupa Sari S Kartasalo Kimmo K Kreutzer Joose J Miettinen Susanna S Kallio Pasi P
Journal of the Royal Society, Interface 20170701 132
Polydimethylsiloxane (PDMS) is widely used in dynamic biological microfluidic applications. As a highly hydrophobic material, native PDMS does not support cell attachment and culture, especially in dynamic conditions. Previous covalent coating methods use glutaraldehyde (GA) which, however, is cytotoxic. This paper introduces a novel and simple method for binding collagen type I covalently on PDMS using ascorbic acid (AA) as a cross-linker instead of GA. We compare the novel method against physi ...[more]