Ontology highlight
ABSTRACT:
SUBMITTER: Ng S
PROVIDER: S-EPMC5553193 | biostudies-literature | 2015 Apr
REPOSITORIES: biostudies-literature
Ng Simon S Lin Edith E Kitov Pavel I PI Tjhung Katrina F KF Gerlits Oksana O OO Deng Lu L Kasper Brian B Sood Amika A Paschal Beth M BM Zhang Ping P Ling Chang-Chun CC Klassen John S JS Noren Christopher J CJ Mahal Lara K LK Woods Robert J RJ Coates Leighton L Derda Ratmir R
Journal of the American Chemical Society 20150416 16
We describe an approach to accelerate the search for competitive inhibitors for carbohydrate-recognition domains (CRDs). Genetically encoded fragment-based discovery (GE-FBD) uses selection of phage-displayed glycopeptides to dock a glycan fragment at the CRD and guide selection of synergistic peptide motifs adjacent to the CRD. Starting from concanavalin A (ConA), a mannose (Man)-binding protein, as a bait, we narrowed a library of 10(8) glycopeptides to 86 leads that share a consensus motif, M ...[more]