Ontology highlight
ABSTRACT:
SUBMITTER: Fruh V
PROVIDER: S-EPMC4104428 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
Chemistry & biology 20100801 8
Membrane proteins are important pharmaceutical targets, but they pose significant challenges for fragment-based drug discovery approaches. Here, we present the first successful use of biophysical methods to screen for fragment ligands to an integral membrane protein. The Escherichia coli inner membrane protein DsbB was solubilized in detergent micelles and lipid bilayer nanodiscs. The solubilized protein was immobilized with retention of functionality and used to screen 1071 drug fragments for b ...[more]