Activated Ion-Electron Transfer Dissociation Enables Comprehensive Top-Down Protein Fragmentation.
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ABSTRACT:
SUBMITTER: Riley NM
PROVIDER: S-EPMC5555583 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Publications
Activated Ion-Electron Transfer Dissociation Enables Comprehensive Top-Down Protein Fragmentation.
Riley Nicholas M NM Westphall Michael S MS Coon Joshua J JJ
Journal of proteome research 20170619 7
Here we report the first demonstration of near-complete sequence coverage of intact proteins using activated ion-electron transfer dissociation (AI-ETD), a method that leverages concurrent infrared photoactivation to enhance electron-driven dissociation. AI-ETD produces mainly c/z-type product ions and provides comprehensive (77-97%) protein sequence coverage, outperforming HCD, ETD, and EThcD for all proteins investigated. AI-ETD also maintains this performance across precursor ion charge state ...[more]
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