Ontology highlight
ABSTRACT:
SUBMITTER: Forster F
PROVIDER: S-EPMC555690 | biostudies-literature | 2005 Mar
REPOSITORIES: biostudies-literature
Förster Friedrich F Medalia Ohad O Zauberman Nathan N Baumeister Wolfgang W Fass Deborah D
Proceedings of the National Academy of Sciences of the United States of America 20050317 13
We used cryo-electron tomography in conjunction with single-particle averaging techniques to study the structures of frozen-hydrated envelope glycoprotein (Env) complexes on intact Moloney murine leukemia retrovirus particles. Cryo-electron tomography allows 3D imaging of viruses in toto at a resolution sufficient to locate individual macromolecules, and local averaging of abundant complexes substantially improves the resolution. The averaging of repetitive features in electron tomograms is hamp ...[more]