Ontology highlight
ABSTRACT:
SUBMITTER: Vijayakrishnan S
PROVIDER: S-EPMC7572381 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Vijayakrishnan Swetha S McElwee Marion M Loney Colin C Rixon Frazer F Bhella David D
Scientific reports 20201019 1
Cryo electron microscopy (cryo-EM), a key method for structure determination involves imaging purified material embedded in vitreous ice. Images are then computationally processed to obtain three-dimensional structures approaching atomic resolution. There is increasing interest in extending structural studies by cryo-EM into the cell, where biological structures and processes may be imaged in context. The limited penetrating power of electrons prevents imaging of thick specimens (> 500 nm) howev ...[more]