Ontology highlight
ABSTRACT:
SUBMITTER: Shen Q
PROVIDER: S-EPMC5557916 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Shen Qingya Q Tan Hongwei H Xing Guo-Wen GW Zheng Jimin J Jia Zongchao Z
Scientific reports 20170815 1
YhdE is a Maf (multicopy associated filamentation) proteins from Escherichia coli which exhibits pyrophosphatase activity towards selected nucleotides, although its catalytic mechanism remains unclear. Herein we used a novel fluorescence probe (4-isoACBA-Zn(II) complex) to characterize the enzymatic properties of YhdE and its mutant, establishing a new method for assaying pyrophosphatase catalytic function. Our results reveal for the first time that the new fluorescence sensor confers high sensi ...[more]