Dataset Information

Multigenerational effects of copper nanomaterials (CuONMs) are different of those of CuCl₂: exposure in the soil invertebrate Enchytraeus crypticus.

ABSTRACT: Nanomaterials (NMs) are recommended to be tested in longer term exposures. Multigenerational (MG) studies are scarce and particularly important because effects can be transferred to the next generation. The current risk assessment framework does not include MG effects and this is a caveat for persistent materials. Here, the effects of copper NMs (CuONMs) and copper salt (CuCl₂) were assessed in a MG exposure (4 generations in spiked soil + 2 generations in clean soil, F1 to F7 generations in total), with the standard soil model Enchytraeus crypticus, using relevant reproduction test effect concentrations (EC₁₀, EC₅₀), monitoring survival and reproduction. This represented ca. 1 year continuous exposure tests. MG effects varied with effect concentration and test materials: CuONMs caused increased toxicity for EC₁₀ exposed organisms (EC₅₀ did not change), and transfer to clean media reset effects, whereas CuCl₂ reduced toxicity for EC₁₀ and EC₅₀, but the transfer to clean media "revived" the initial effects, i.e. close to EC₅₀ levels in F7. Clearly CuONMs and CuCl₂ cause different mechanisms of toxicity or response in the long term, not predictable based on short term or one generation studies. The present contributes for the improvement of risk assessment, adding important information for the long term exposure and effects.

SUBMITTER: Bicho RC

PROVIDER: S-EPMC5559477 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Multigenerational effects of copper nanomaterials (CuONMs) are different of those of CuCl<sub>2</sub>: exposure in the soil invertebrate Enchytraeus crypticus.

Bicho Rita C RC Santos Fátima C F FCF Scott-Fordsmand Janeck J JJ Amorim Mónica J B MJB

Scientific reports 20170816 1

Nanomaterials (NMs) are recommended to be tested in longer term exposures. Multigenerational (MG) studies are scarce and particularly important because effects can be transferred to the next generation. The current risk assessment framework does not include MG effects and this is a caveat for persistent materials. Here, the effects of copper NMs (CuONMs) and copper salt (CuCl<sub>2</sub>) were assessed in a MG exposure (4 generations in spiked soil + 2 generations in clean soil, F1 to F7 generat ...[more]

PMID: 28814818

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Project description:Polycyclic Aromatic Hydrocarbons (PAHs) continue to cause environmental challenges due to their release in the environment by a great variety of anthropogenic activities and their accumulation in soil ecosystems. Here we studied the toxicological effect of the model PAH phenanthrene (Phe) on the soil invertebrate model Enchytraeus crypticus at the individual, tissue and molecular level. Organisms were exposed to Phe for 2 and 21 days to the (previously estimated) EC10 and EC50 (population reproduction over 3 weeks). Gene expression profiling did not reveal a typical Phe-induced biotransfor-mation signature, as it usually does in arthropods and vertebrates. Instead, we observed only general metabolic processes to be affected after 2 days of exposure, such as translation and ATP synthesis-coupled electron transport. Histological sections of tissues of 2-day exposed animals did not show any deviations from the control situation. In contrast, prolonged exposure up to 21 days showed histopathological effects: chloragogenous cells were highly vacuolated and hypertrophic. This was corroborated by differential expression of genes related to immune response and oxidative stress at the transcriptomic level. The data exemplify the complexity and species-specific features of PAH toxicity among soil invertebrate communities, which restricts read-across and extrapolation in the context of soil ecological risk assessment. The data presented in our manuscript is an exposure experiment where E. cryticus is exposed to phenanthrene EC10 and EC50 on reproduction for 2 and 21 days. A single channel, interwoven loop design was used to test animals. 4 biological replicates per condition were used containing 25 grams of soil and 5 - 7, adult old animals per replicate. The platform is a 4*180k Agilent platform containing some 86k E. crypticus probes in duplicate. However, only a subset of the probes (23k) was used for the analysis. To see which probes were used in the analysis see the raw data files control type column, only probes which are denoted with a 0 were used.

Dataset Information

Multigenerational effects of copper nanomaterials (CuONMs) are different of those of CuCl₂: exposure in the soil invertebrate Enchytraeus crypticus.

Publications

Multigenerational effects of copper nanomaterials (CuONMs) are different of those of CuCl<sub>2</sub>: exposure in the soil invertebrate Enchytraeus crypticus.

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Dataset Information

Multigenerational effects of copper nanomaterials (CuONMs) are different of those of CuCl2: exposure in the soil invertebrate Enchytraeus crypticus.

Publications

Multigenerational effects of copper nanomaterials (CuONMs) are different of those of CuCl<sub>2</sub>: exposure in the soil invertebrate Enchytraeus crypticus.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Multigenerational effects of copper nanomaterials (CuONMs) are different of those of CuCl₂: exposure in the soil invertebrate Enchytraeus crypticus.