Mass Cytometric Analysis of HIV Entry, Replication, and Remodeling in Tissue CD4+ T Cells.
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ABSTRACT:
SUBMITTER: Cavrois M
PROVIDER: S-EPMC5560086 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Publications
Mass Cytometric Analysis of HIV Entry, Replication, and Remodeling in Tissue CD4+ T Cells.
Cavrois Marielle M Banerjee Trambak T Mukherjee Gourab G Raman Nandhini N Hussien Rajaa R Rodriguez Brandon Aguilar BA Vasquez Joshua J Spitzer Matthew H MH Lazarus Nicole H NH Jones Jennifer J JJ Ochsenbauer Christina C McCune Joseph M JM Butcher Eugene C EC Arvin Ann M AM Sen Nandini N Greene Warner C WC Roan Nadia R NR
Cell reports 20170701 4
To characterize susceptibility to HIV infection, we phenotyped infected tonsillar T cells by single-cell mass cytometry and created comprehensive maps to identify which subsets of CD4+ T cells support HIV fusion and productive infection. By comparing HIV-fused and HIV-infected cells through dimensionality reduction, clustering, and statistical approaches to account for viral perturbations, we identified a subset of memory CD4+ T cells that support HIV entry but not viral gene expression. These c ...[more]
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