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Mechanically Induced Catalytic Amplification Reaction for Readout of Receptor-Mediated Cellular Forces.


ABSTRACT: Mechanics play a fundamental role in cell biology, but detecting piconewton (pN) forces is challenging because of a lack of accessible and high throughput assays. A mechanically induced catalytic amplification reaction (MCR) for readout of receptor-mediated forces in cells is described. Mechanically labile DNA duplexes presenting ligands are surface immobilized such that specific receptor forces denature the duplex and thus expose a blocked primer. Amplification of primers is achieved using an isothermal polymerization reaction and quantified by fluorescence readout. As a proof of concept, the assay was used to test the activity of a mechanomodulatory drug and integrin adhesion receptor antibodies. To the best of our knowledge, this is the first example of a catalytic reaction triggered in response to molecular piconewton forces. The MCR may transform the field of mechanobiology by providing a new facile tool to detect receptor specific mechanics with the convenience of the polymerase chain reaction (PCR).

SUBMITTER: Ma VP 

PROVIDER: S-EPMC5563213 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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Mechanically Induced Catalytic Amplification Reaction for Readout of Receptor-Mediated Cellular Forces.

Ma Victor Pui-Yan VP   Liu Yang Y   Yehl Kevin K   Galior Kornelia K   Zhang Yun Y   Salaita Khalid K  

Angewandte Chemie (International ed. in English) 20160401 18


Mechanics play a fundamental role in cell biology, but detecting piconewton (pN) forces is challenging because of a lack of accessible and high throughput assays. A mechanically induced catalytic amplification reaction (MCR) for readout of receptor-mediated forces in cells is described. Mechanically labile DNA duplexes presenting ligands are surface immobilized such that specific receptor forces denature the duplex and thus expose a blocked primer. Amplification of primers is achieved using an i  ...[more]

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