Ontology highlight
ABSTRACT:
SUBMITTER: Ma VP
PROVIDER: S-EPMC5563213 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Ma Victor Pui-Yan VP Liu Yang Y Yehl Kevin K Galior Kornelia K Zhang Yun Y Salaita Khalid K
Angewandte Chemie (International ed. in English) 20160401 18
Mechanics play a fundamental role in cell biology, but detecting piconewton (pN) forces is challenging because of a lack of accessible and high throughput assays. A mechanically induced catalytic amplification reaction (MCR) for readout of receptor-mediated forces in cells is described. Mechanically labile DNA duplexes presenting ligands are surface immobilized such that specific receptor forces denature the duplex and thus expose a blocked primer. Amplification of primers is achieved using an i ...[more]