Microfluidic perfusion shows intersarcomere dynamics within single skeletal muscle myofibrils.

ABSTRACT: The sarcomere is the smallest functional unit of myofibrils in striated muscles. Sarcomeres are connected in series through a network of elastic and structural proteins. During myofibril activation, sarcomeres develop forces that are regulated through complex dynamics among their structures. The mechanisms that regulate intersarcomere dynamics are unclear, which limits our understanding of fundamental muscle features. Such dynamics are associated with the loss in forces caused by mechanical instability encountered in muscle diseases and cardiomyopathy and may underlie potential target treatments for such conditions. In this study, we developed a microfluidic perfusion system to control one sarcomere within a myofibril, while measuring the individual behavior of all sarcomeres. We found tha

SUBMITTER: de Souza Leite F 

PROVIDER: S-EPMC5565411 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature