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Conformational and chemical selection by a trans-acting editing domain.


ABSTRACT: Molecular sieves ensure proper pairing of tRNAs and amino acids during aminoacyl-tRNA biosynthesis, thereby avoiding detrimental effects of mistranslation on cell growth and viability. Mischarging errors are often corrected through the activity of specialized editing domains present in some aminoacyl-tRNA synthetases or via single-domain trans-editing proteins. ProXp-ala is a ubiquitous trans-editing enzyme that edits Ala-tRNAPro, the product of Ala mischarging by prolyl-tRNA synthetase, although the structural basis for discrimination between correctly charged Pro-tRNAPro and mischarged Ala-tRNAAla is unclear. Deacylation assays using substrate analogs reveal that size discrimination is only one component of selectivity. We used NMR spectroscopy and sequence conservation to guide extensive site-directed mutagenesis of Caulobacter crescentus ProXp-ala, along with binding and deacylation assays to map specificity determinants. Chemical shift perturbations induced by an uncharged tRNAPro acceptor stem mimic, microhelixPro, or a nonhydrolyzable mischarged Ala-microhelixPro substrate analog identified residues important for binding and deacylation. Backbone 15N NMR relaxation experiments revealed dynamics for a helix flanking the substrate binding site in free ProXp-ala, likely reflecting sampling of open and closed conformations. Dynamics persist on binding to the uncharged microhelix, but are attenuated when the stably mischarged analog is bound. Computational docking and molecular dynamics simulations provide structural context for these findings and predict a role for the substrate primary ?-amine group in substrate recognition. Overall, our results illuminate strategies used by a trans-editing domain to ensure acceptance of only mischarged Ala-tRNAPro, including conformational selection by a dynamic helix, size-based exclusion, and optimal positioning of substrate chemical groups.

SUBMITTER: Danhart EM 

PROVIDER: S-EPMC5565427 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Conformational and chemical selection by a <i>trans</i>-acting editing domain.

Danhart Eric M EM   Bakhtina Marina M   Cantara William A WA   Kuzmishin Alexandra B AB   Ma Xiao X   Sanford Brianne L BL   Vargas-Rodriguez Oscar O   Košutić Marija M   Goto Yuki Y   Suga Hiroaki H   Nakanishi Kotaro K   Micura Ronald R   Foster Mark P MP   Musier-Forsyth Karin K  

Proceedings of the National Academy of Sciences of the United States of America 20170802 33


Molecular sieves ensure proper pairing of tRNAs and amino acids during aminoacyl-tRNA biosynthesis, thereby avoiding detrimental effects of mistranslation on cell growth and viability. Mischarging errors are often corrected through the activity of specialized editing domains present in some aminoacyl-tRNA synthetases or via single-domain <i>trans</i>-editing proteins. ProXp-ala is a ubiquitous <i>trans</i>-editing enzyme that edits Ala-tRNA<sup>Pro</sup>, the product of Ala mischarging by prolyl  ...[more]

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