The molecular chaperones DNAJB6 and Hsp70 cooperate to suppress ?-synuclein aggregation.

ABSTRACT: A major hallmark of Parkinson's disease (PD) is the presence of Lewy bodies (LBs) in certain neuronal tissues. LBs are protein-rich inclusions, in which ?-synuclein (?-syn) is the most abundant protein. Since these inclusions are not present in healthy individuals, despite the high concentration of ?-syn in neurons, it is important to investigate whether natural control mechanisms are present to efficiently suppress ?-syn aggregation. Here, we demonstrate that a CRISPR/Cas9-mediated knockout (KO) of a DnaJ protein, DNAJB6, in HEK293T cells expressing ?-syn, causes a massive increase in ?-syn aggregation. Upon DNAJB6 re-introduction into these DNAJB6-KO HEK293T-?-syn cells, aggregation is reduced to the level of the parental cells. We then show that the suppression of ?-syn aggregation is dependent on the J-domain of DNAJB6, as the catalytically inactive protein, which carries the H31Q mutation, does not suppress aggregation, when re-introduced into DNAJB6-KO cells. We further demonstrate, that the suppression of ?-syn aggregation is dependent on the molecular chaperone Hsp70, which is consistent with the well-known function of J-domains of transferring unfolded and misfolded proteins to Hsp70. These data identify a natural control strategy to suppress ?-syn aggregation and suggest potential therapeutic approaches to prevent or treat PD and related disorders.

SUBMITTER: Aprile FA 

PROVIDER: S-EPMC5567236 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature