Transcription-Replication Conflict Orientation Modulates R-Loop Levels and Activates Distinct DNA Damage Responses.
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ABSTRACT:
SUBMITTER: Hamperl S
PROVIDER: S-EPMC5570545 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Publications
Transcription-Replication Conflict Orientation Modulates R-Loop Levels and Activates Distinct DNA Damage Responses.
Hamperl Stephan S Bocek Michael J MJ Saldivar Joshua C JC Swigut Tomek T Cimprich Karlene A KA
Cell 20170801 4
Conflicts between transcription and replication are a potent source of DNA damage. Co-transcriptional R-loops could aggravate such conflicts by creating an additional barrier to replication fork progression. Here, we use a defined episomal system to investigate how conflict orientation and R-loop formation influence genome stability in human cells. R-loops, but not normal transcription complexes, induce DNA breaks and orientation-specific DNA damage responses during conflicts with replication fo ...[more]
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