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Pseudomonas aeruginosa Clinical Isolates in Nepal Coproducing Metallo-?-Lactamases and 16S rRNA Methyltransferases.

ABSTRACT: A total of 11 multidrug-resistant Pseudomonas aeruginosa clinical isolates were obtained in Nepal. Four of these isolates harbored genes encoding one or more carbapenemases (DIM-1, NDM-1, and/or VIM-2), and five harbored genes encoding a 16S rRNA methyltransferase (RmtB4 or RmtF2). A novel RmtF variant, RmtF2, had a substitution (K65E) compared with the same gene in RmtF. To our knowledge, this is the first report describing carbapenemase- and 16S rRNA methyltransferase-coproducing P. aeruginosa clinical isolates in Nepal.

SUBMITTER: Tada T

PROVIDER: S-EPMC5571325 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Pseudomonas aeruginosa Clinical Isolates in Nepal Coproducing Metallo-β-Lactamases and 16S rRNA Methyltransferases.

Tada Tatsuya T Shimada Kayo K Satou Kazuhito K Hirano Takashi T Pokhrel Bharat M BM Sherchand Jeevan B JB Kirikae Teruo T

Antimicrobial agents and chemotherapy 20170824 9

A total of 11 multidrug-resistant <i>Pseudomonas aeruginosa</i> clinical isolates were obtained in Nepal. Four of these isolates harbored genes encoding one or more carbapenemases (DIM-1, NDM-1, and/or VIM-2), and five harbored genes encoding a 16S rRNA methyltransferase (RmtB4 or RmtF2). A novel RmtF variant, RmtF2, had a substitution (K65E) compared with the same gene in RmtF. To our knowledge, this is the first report describing carbapenemase- and 16S rRNA methyltransferase-coproducing <i>P. ...[more]

PMID: 28696242

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Isolation of Pseudomonas aeruginosa coproducing metallo-β-lactamase SPM-1 and 16S rRNA methylase RmtD1 in an urban river.

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| S-EPMC3101456 | biostudies-literature

NDM-1 Metallo-β-Lactamase and ArmA 16S rRNA methylase producing Providencia rettgeri clinical isolates in Nepal.

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| S-EPMC3922589 | biostudies-literature

Detection of Metallo-Beta Lactamases Among Carbapenem-Resistant Pseudomonas aeruginosa.

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Emergence and Spread of Carbapenem-Resistant and Aminoglycoside-Panresistant Enterobacter cloacae Complex Isolates Coproducing NDM-Type Metallo-?-Lactamase and 16S rRNA Methylase in Myanmar.

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Emergence of 16S rRNA methylase-producing Acinetobacter baumannii and Pseudomonas aeruginosa isolates in hospitals in Vietnam.

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| S-EPMC3680199 | biostudies-literature

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| S-EPMC3770254 | biostudies-literature

Coproduction of novel 16S rRNA methylase RmtD and metallo-beta-lactamase SPM-1 in a panresistant Pseudomonas aeruginosa isolate from Brazil.

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| S-EPMC2493107 | biostudies-literature

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| S-EPMC3553699 | biostudies-literature

Detection of bla(IMP) and bla(VIM) metallo-β-lactamases genes among Pseudomonas aeruginosa strains.

Project description:Acquired Metallo-β-Lactamases (MBLs) are emerging resistance determinants in Pseudomonas aeruginosa and other gram-negative bacteria.Using Combination Disk Diffusion test, it was found that among 83 imipenem non-susceptible P. aeruginosa strains, 48 (57.9%) were MBL producers. PCR and Sequencing methods proved that these isolates were positive for blaIMP-1 genes, whereas none were positive for bla(VIM) genes. The mortality rate due to MBL-producing Pseudomonas infection was 4 (8.3%) among the hospitalized patients. Therefore, identification of drug resistance patterns in P. aeruginosa and detection of MBLs producing isolates are of great importance in the prevention and control of infections.

| S-EPMC3636667 | biostudies-literature

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