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Transcription controls growth, cell kinetics and cholesterol supply to sustain ACTH responses.

ABSTRACT: Chronic ACTH exposure is associated with adrenal hypertrophy and steroidogenesis. The underlying molecular processes in mice have been analysed by microarray, histological and immunohistochemical techniques. Synacthen infused for 2 weeks markedly increased adrenal mass and plasma corticosterone levels. Microarray analysis found greater than 2-fold changes in expression of 928 genes (P?4-fold and cross-sectional area of fasciculata cells was 2-fold greater. In contrast, genes associated with apoptosis (eg Casp12, Clu,) were downregulated and apoptotic cells (Tunel staining) were fewer (P?

SUBMITTER: Menzies RI

PROVIDER: S-EPMC5574282 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Transcription controls growth, cell kinetics and cholesterol supply to sustain ACTH responses.

Menzies Robert I RI Zhao Xin X Mullins Linda J LJ Mullins John J JJ Cairns Carolynn C Wrobel Nicola N Dunbar Donald R DR Bailey Matthew A MA Kenyon Christopher J CJ

Endocrine connections 20170718 7

Chronic ACTH exposure is associated with adrenal hypertrophy and steroidogenesis. The underlying molecular processes in mice have been analysed by microarray, histological and immunohistochemical techniques. Synacthen infused for 2 weeks markedly increased adrenal mass and plasma corticosterone levels. Microarray analysis found greater than 2-fold changes in expression of 928 genes (<i>P</i> < 0.001; 397 up, 531 down). These clustered in pathways involved in signalling, sterol/lipid metabolism, ...[more]

PMID: 28720595

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