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Cpf1 proteins excise CRISPR RNAs from mRNA transcripts in mammalian cells.

ABSTRACT: Cpf1 is a CRISPR effector protein that has greater specificity than Streptococcus pyogenes Cas9 (SpCas9) in genome-editing applications. Here we show that Lachnospiraceae bacterium (Lb) and Acidaminococus sp. (As) Cpf1 orthologs have RNase activities that can excise multiple CRISPR RNAs (crRNAs) from a single RNA polymerase II-driven RNA transcript expressed in mammalian cells. This property simplifies modification of multiple genomic targets and can be used to increase the efficiency of Cpf1-mediated editing.

SUBMITTER: Zhong G

PROVIDER: S-EPMC5577360 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Cpf1 proteins excise CRISPR RNAs from mRNA transcripts in mammalian cells.

Zhong Guocai G Wang Haimin H Li Yujun Y Tran Mai H MH Farzan Michael M

Nature chemical biology 20170619 8

Cpf1 is a CRISPR effector protein that has greater specificity than Streptococcus pyogenes Cas9 (SpCas9) in genome-editing applications. Here we show that Lachnospiraceae bacterium (Lb) and Acidaminococus sp. (As) Cpf1 orthologs have RNase activities that can excise multiple CRISPR RNAs (crRNAs) from a single RNA polymerase II-driven RNA transcript expressed in mammalian cells. This property simplifies modification of multiple genomic targets and can be used to increase the efficiency of Cpf1-me ...[more]

PMID: 28628097

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