Dataset Information

Evaluation of stress effects of copper oxide nanoparticles in Brassica napus L. seedlings.

ABSTRACT: Rapid growth of nanotechnology has enabled the production and use of engineered nanoparticles (ENPs) in several industries as well as in agriculture areas. This has raised ecotoxicological concerns due to the release of ENPs to the environment. In the present study, we investigated the effects of interactions of copper oxide nanoparticles (CuO NPs) on physiological, biochemical, and molecular indices in seedlings of an important oil seed crop Brassica napus L. The seedlings were treated with 0, 20, 50, 100, 200, 400, and 500 mg/L of CuO NPs for 14 days in half-strength semi-solid Murashige and Skoog medium. The CuO NPs treatment significantly reduced shoot and root growth as well as plant biomass. Shortening and thickening of primary and lateral roots and inhibition of lateral root growth was observed at higher concentrations. An increase in reactive oxygen species generation, and malondialdehyde accumulation was observed. Histochemical staining of roots with propidium iodide and aniline blue indicated cell death and callose formation in roots. Transcriptional modulation of genes related to oxidative stress viz. CuZn superoxide dismutase, catalase, and ascorbate peroxidase was observed. Element content analysis showed an increase in Cu content and decrease in Fe, Mn, and Zn contents. Overall, exposure to CuO NPs caused oxidative injury, cell death, callose formation, and decreased the micro nutrient contents in B. napus seedlings.

SUBMITTER: Nair PMG

PROVIDER: S-EPMC5577373 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Evaluation of stress effects of copper oxide nanoparticles in <i>Brassica napus</i> L. seedlings.

Nair Prakash M Gopalakrishnan PMG Chung Ill Min IM

3 Biotech 20170830 5

Rapid growth of nanotechnology has enabled the production and use of engineered nanoparticles (ENPs) in several industries as well as in agriculture areas. This has raised ecotoxicological concerns due to the release of ENPs to the environment. In the present study, we investigated the effects of interactions of copper oxide nanoparticles (CuO NPs) on physiological, biochemical, and molecular indices in seedlings of an important oil seed crop <i>Brassica napus</i> L. The seedlings were treated w ...[more]

PMID: 28868220

Similar Datasets

Project description:In this paper, the effect of isosteviol on the physiological metabolism of Brassica napus seedlings under salt stress is explored. Brassica napus seeds (Qinyou 2) were used as materials, and the seeds were soaked in different concentrations of isosteviol under salt stress. The fresh weight, dry weight, osmotic substance, absorption and distribution of Na+, K+, Cl-, and the content of reactive oxygen species (ROS) were measured, and these results were combined with the changes shown by Fourier transform infrared spectroscopy (FTIR). The results showed that isosteviol at an appropriate concentration could effectively increase the biomass and soluble protein content of Brassica napus seedlings and reduce the contents of proline, glycine betaine, and ROS in the seedlings. Isosteviol reduces the oxidative damage to Brassica napus seedlings caused by salt stress by regulating the production of osmotic substances and ROS. In addition, after seed soaking in isosteviol, the Na+ content in the shoots of the Brassica napus seedlings was always lower than that in the roots, while the opposite was true for the K+ content. This indicated that under salt stress the Na+ absorbed by the Brassica napus seedlings was mainly accumulated in the roots and that less Na+ was transported to the shoots, while more of the K+ absorbed by the Brassica napus seedlings was retained in the leaves. It is speculated that this may be an important mechanism for Brassica napus seedlings to relieve Na+ toxicity. The spectroscopy analysis showed that, compared with the control group (T1), salt stress increased the absorbance values of carbohydrates, proteins, lipids, nucleic acids, etc., indicating structural damage to the plasma membrane and cell wall. The spectra of the isosteviol seed soaking treatment group were nearly the same as those of the control group (T1). The correlation analysis shows that under salt stress the Brassica napus seedling tissues could absorb large amounts of Na+ and Cl- to induce oxidative stress and inhibit the growth of the plants. After the seed soaking treatment, isosteviol could significantly reduce the absorption of Na+ by the seedling tissues, increase the K+ content, and reduce the salt stress damage to the plant seedlings. Therefore, under salt stress, seed soaking with isosteviol at an appropriate concentration (10-9~10-8 M) can increase the salt resistance of Brassica napus seedlings by regulating their physiological and metabolic functions.

Project description:Cold stress (CS) severely affects several physiological, biochemical, and molecular mechanisms and limits the growth and production of rapeseed (Brassica napus L.). Trehalose (Tre) acts as a growth modulator, which is extensively used to improve the tolerance to multiple plant stresses. Further, Tre also serves as an external force in inducing plant signaling molecules, regulating the expression of stress-responsive genes, and enhancing the CS tolerance in plants. Nevertheless, the importance of exogenous Tre in improving the CS tolerance in rapeseed is still unclear. Therefore, the current study was designed to get mechanistic insights into Tre-mediated CS tolerance in rapeseed seedlings. To explore the Tre role, we designed four treatments [control (CK), CK + 20 mM L-1 Tre, Cold, and Cold + 20 mM L-1 Tre] and three CS conditions (4, 0, and -4°C). The results showed that Tre treatments significantly mitigated the adverse effects of CS on the seedlings and increased the survival rate of Tre-treated seedlings under CS conditions. The exogenous Tre dramatically increased the contents of osmoprotectants, including the soluble sugar (SS), soluble protein (SP), and proline (Pro), and the activities of antioxidant enzymes, such as catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), and ascorbate peroxidase (APX) were also increased under CS conditions. Additionally, Tre decreased the malondialdehyde (MDA) contents to protect the rapeseed seedlings. Moreover, Tre also remarkably augmented the expression levels of antioxidant genes (CAT12, POD34, and FSD7), CS-responsive marker genes (CBF1, CBF2, CBF4, COR6.6, COR15, COR25, COL1, and KIN1), and Tre-biosynthesis genes (TPS4, TPS8, and TPS9). Briefly, exogenous Tre not only regulates the antioxidant and osmotic balance, but it also significantly participates in Tre metabolism and signaling network to improve the CS tolerance in rapeseed. Thus, Tre-induced supervisory connections between physiological or/and biochemical attributes provide information to dissect the mechanisms of Tre-mediated CS tolerance.

Dataset Information

Evaluation of stress effects of copper oxide nanoparticles in Brassica napus L. seedlings.

Publications

Evaluation of stress effects of copper oxide nanoparticles in <i>Brassica napus</i> L. seedlings.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets