Dataset Information

Fourteen simple-sequence repeats newly developed for population genetic studies in Prosopis africana (Fabaceae-Mimosoideae).

ABSTRACT: There is very limited genetic knowledge in Prosopis africana, an important sub-Saharan multi-purpose tree species. Availability of highly polymorphic genetic markers would be helpful for future genetic work.Leaf samples from 15 trees were used to develop simple sequence repeat (SSR) markers. Size-selected fragments from genomic DNA were enriched for repeats and the library was analyzed on an Illumina MiSeq platform. Fourteen SSRs were selected and applied in two Burkinabe populations (40 adult trees each). The number of alleles varied from 4 to 20, evenness (effective number of alleles/observed number of alleles) averaged to 0.54 and unbiased heterozygosity ranged from 0.305 to 0.925 over all loci and populations. Null alleles were not detected.Due to the high level of polymorphism and lack of null alleles the developed SSRs can be effectively employed in population genetic studies.

SUBMITTER: Zerbo GC

PROVIDER: S-EPMC5580295 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Fourteen simple-sequence repeats newly developed for population genetic studies in Prosopis africana (Fabaceae-Mimosoideae).

Zerbo Guibien Cleophas GC Konrad Heino H Ouedraogo Moussa M Geburek Thomas T

BMC research notes 20170831 1

<h4>Background</h4>There is very limited genetic knowledge in Prosopis africana, an important sub-Saharan multi-purpose tree species. Availability of highly polymorphic genetic markers would be helpful for future genetic work.<h4>Findings</h4>Leaf samples from 15 trees were used to develop simple sequence repeat (SSR) markers. Size-selected fragments from genomic DNA were enriched for repeats and the library was analyzed on an Illumina MiSeq platform. Fourteen SSRs were selected and applied in t ...[more]

PMID: 28859692

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Project description:BackgroundThe length of stay (LOS) in intensive care units (ICUs) has been used as a good indicator not only for resource consumption but also for health outcomes of patients. However, data regarding pediatric LOS in Japanese ICUs are limited. The primary aim of this study was to characterize the Japanese pediatric ICU patients based on their LOS. Second, we aimed to develop a simple scoring system to predict long-stay pediatric ICU patients on admission.MethodsWe performed a retrospective cohort study using consecutive pediatric data (aged <?16?years) registered in the Japanese Registry of Pediatric Acute Care (JaRPAC) from October 2013 to September 2016, which consisted of descriptive and diagnostic information. The factors for long-stay patients (LSPs; LOS?>?14?days) were identified using multiple regression analysis, and subsequently, a simple predictive scoring system was developed based on the results. The validity of the score was prospectively tested using data from the JaRPAC registration from October 2016 to September 2017.ResultsOverall, 4107 patients were included. Although LSPs were few (8.0% [n =?330]), they consumed 38.0% of ICU bed days (9750 for LSPs versus 25,659 overall). Mortality was seven times higher in LSPs than in short-stay patients (9.1% versus 1.3%). An 11-variable simple predictive scoring system was constructed, including Pediatric Index of Mortality 2???1 (2 points), liver dysfunction (non-post operation) (2 points), post-cardiopulmonary resuscitation (1 point), circulatory disorder (1 point), post-operative management of liver transplantation (1 point), encephalitis/encephalopathy (1 point), myocarditis/cardiomyopathy (1 point), congenital heart disease (non-post operation) (1 point), lung tissue disease (1 point), Pediatric Cerebral Performance Category scores ??2 (1 point), and age <?2?years (1 point). A score of ??3 points yielded an area under the receiver operating characteristic curve (AUC) of 0.79, sensitivity of 87.0%, and specificity of 59.4% in the original dataset. Reproducibility was confirmed with the internal validation dataset (AUC 0.80, sensitivity 92.6%, and specificity 60.2%).ConclusionsPediatric LSPs possess a significant presence in Japanese ICUs with high rates of bed utilization and mortality. The newly developed predictive scoring system may identify pediatric LSPs on admission.

Dataset Information

Fourteen simple-sequence repeats newly developed for population genetic studies in Prosopis africana (Fabaceae-Mimosoideae).

Publications

Fourteen simple-sequence repeats newly developed for population genetic studies in Prosopis africana (Fabaceae-Mimosoideae).

Similar Datasets

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