Ontology highlight
ABSTRACT:
SUBMITTER: Boothe T
PROVIDER: S-EPMC5582871 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Boothe Tobias T Hilbert Lennart L Heide Michael M Berninger Lea L Huttner Wieland B WB Zaburdaev Vasily V Vastenhouw Nadine L NL Myers Eugene W EW Drechsel David N DN Rink Jochen C JC
eLife 20170714
In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus substantially improves image quality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral organo ...[more]