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A tunable refractive index matching medium for live imaging cells, tissues and model organisms.

ABSTRACT: In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus substantially improves image quality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral organoids.

SUBMITTER: Boothe T

PROVIDER: S-EPMC5582871 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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A tunable refractive index matching medium for live imaging cells, tissues and model organisms.

Boothe Tobias T Hilbert Lennart L Heide Michael M Berninger Lea L Huttner Wieland B WB Zaburdaev Vasily V Vastenhouw Nadine L NL Myers Eugene W EW Drechsel David N DN Rink Jochen C JC

eLife 20170714

In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus substantially improves image quality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral organo ...[more]

PMID: 28708059

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