Unknown

Dataset Information

0

Amentoflavone enhances sorafenib-induced apoptosis through extrinsic and intrinsic pathways in sorafenib-resistant hepatocellular carcinoma SK-Hep1 cells in vitro.


ABSTRACT: The present study aimed to evaluate the effects of amentoflavone on sorafenib-induced apoptosis in sorafenib-resistant hepatocellular carcinoma (HCC) cells. The sorafenib-resistant SK-Hep1 (SK-Hep1R) cell line was established for the present study. Initially, the differences in sorafenib-induced cytotoxicity and apoptosis between wild-type SK-Hep1 and SK-Hep1R cells were verified using the MTT assay and flow cytometry. The effects of amentoflavone on sorafenib-induced cytotoxicity and apoptosis were then investigated using MTT, flow cytometry, DNA gel electrophoresis and western blot analysis. The results demonstrated that cell viability of SK-Hep1R cells was increased compared with that of SK-Hep1 cells following treatment with different concentrations of sorafenib for 24 h. Apoptosis of SK-Hep1R cells was lower than that of SK-Hep1 cells following treatment with 20 µM sorafenib for 24 h. Amentoflavone alone did not inhibit cell viability but significantly triggered sorafenib-induced cytotoxicity and apoptosis in SK-Hep1R cells. Amentoflavone not only reversed sorafenib-induced anti-apoptotic protein levels but also enhanced sorafenib-induced pro-apoptotic protein expression in SK-Hep1R cells. In conclusion, amentoflavone may be used as a sorafenib sensitizer to enhance sorafenib-induced cytotoxicity and trigger sorafenib-induced apoptosis through extrinsic and intrinsic pathways in SK-Hep1R cells.

SUBMITTER: Chen WL 

PROVIDER: S-EPMC5588078 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Amentoflavone enhances sorafenib-induced apoptosis through extrinsic and intrinsic pathways in sorafenib-resistant hepatocellular carcinoma SK-Hep1 cells <i>in vitro</i>.

Chen Wei-Lung WL   Hsieh Chia-Ling CL   Chen Jiann-Hwa JH   Huang Chih-Sheng CS   Chen Wei-Ting WT   Kuo Yu-Cheng YC   Chen Cheng-Yu CY   Hsu Fei-Ting FT  

Oncology letters 20170708 3


The present study aimed to evaluate the effects of amentoflavone on sorafenib-induced apoptosis in sorafenib-resistant hepatocellular carcinoma (HCC) cells. The sorafenib-resistant SK-Hep1 (SK-Hep1R) cell line was established for the present study. Initially, the differences in sorafenib-induced cytotoxicity and apoptosis between wild-type SK-Hep1 and SK-Hep1R cells were verified using the MTT assay and flow cytometry. The effects of amentoflavone on sorafenib-induced cytotoxicity and apoptosis  ...[more]

Similar Datasets

| S-EPMC4457926 | biostudies-literature
| S-EPMC5986898 | biostudies-literature
| S-EPMC4047891 | biostudies-literature
| S-EPMC8706091 | biostudies-literature
2013-04-22 | E-GEOD-37640 | biostudies-arrayexpress
| S-EPMC3311780 | biostudies-literature
| S-EPMC2701179 | biostudies-literature
| S-EPMC7785961 | biostudies-literature
| S-EPMC8582520 | biostudies-literature
| S-EPMC3057777 | biostudies-literature