Recycling of a selectable marker with a self-excisable plasmid in Pichia pastoris.
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ABSTRACT:
SUBMITTER: Li C
PROVIDER: S-EPMC5593967 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Publications
Recycling of a selectable marker with a self-excisable plasmid in Pichia pastoris.
Li Cheng C Lin Ying Y Zheng Xueyun X Yuan Qingyan Q Pang Nuo N Liao Xihao X Huang Yuanyuan Y Zhang Xinying X Liang Shuli S
Scientific reports 20170911 1
Pichia pastoris is a widely used heterologous protein production workhorse. However, with its multiple genetic modifications to solve bottlenecks for heterologous protein productivity, P. pastoris lacks selectable markers. Existing selectable marker recycling plasmids have drawbacks (e.g., slow growth and conditional lethality). Here, zeocin-resistance marker recycling vectors were constructed using the Cre/loxP recombination system. The vectors were used to (i) knock in heterologous phytase, xy ...[more]
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