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Characterization of Echinostoma cinetorchis endoribonuclease, RNase H.

ABSTRACT: Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis endoribonuclease (RNase H; EcRNH) and to elucidate its molecular biological characters. EcRNH consisted of 308 amino acids and showed low similarity to endoribonucleases of other parasites (<40%). EcRNH had an active site centered on a putative DDEED motif instead of DEDD conserved in other species. A recombinant EcRNH produced as a soluble form in Escherichia coli showed enzymatic activity to cleave the 3'-O-P bond of RNA in a DNA-RNA duplex, producing 3'-hydroxyl and 5'-phosphate. These findings may contribute to develop antisense oligonucleotides which could damage echinostomes and other flukes.

SUBMITTER: Lim SB

PROVIDER: S-EPMC5594731 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Characterization of Echinostoma cinetorchis endoribonuclease, RNase H.

Lim Sung-Bin SB Cha Seok Ho SH Jegal Seung S Jun Hojong H Park Seo Hye SH Jeon Bo-Young BY Pak Jhang Ho JH Bakh Young Yil YY Kim Tong-Soo TS Lee Hyeong-Woo HW

The Korean journal of parasitology 20170831 4

Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis endoribonuclease (RNase H; EcRNH) and to elucidate its molecular biological characters. EcRNH consisted of 308 amino acids and showed low similarity to endoribonucleases of other parasites (<40%). EcRNH had an active site centered on a putative DDEED motif instead of DEDD conserved in other spe ...[more]

PMID: 28877580

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