MALDI-TOF-MS based identification and molecular characterization of food associated methicillin-resistant Staphylococcus aureus.

ABSTRACT: Food-borne methicillin resistant Staphylococcus aureus (MRSA) is involved in two-fold higher mortality rate compared to methicillin susceptible S. aureus (MSSA). Eventhough Mysuru recognized as cleanest city in the world, prevalence of food contamination is not detailed. The aim is to screen food samples from Mysuru area and to characterize MRSA strain, employing MALDI-Biotyper, multiplex PCR to distinguish between MRSA and MSSA by PCR-coupled single strand conformation polymorphism (PCR-SSCP). Of all the food-borne pathogens, S. aureus contamination accounts for 94.37?±?0.02% (P?99%), robust (<2?min), and alternative tool for pathogen identification, and we developed a PCR-SSCP technique for rapid detection of MSSA and MRSA strains.

SUBMITTER: Manukumar HM 

PROVIDER: S-EPMC5595867 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature