Ontology highlight
ABSTRACT:
SUBMITTER: Sdano MA
PROVIDER: S-EPMC5599234 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Sdano Matthew A MA Fulcher James M JM Palani Sowmiya S Chandrasekharan Mahesh B MB Parnell Timothy J TJ Whitby Frank G FG Formosa Tim T Hill Christopher P CP
eLife 20170816
We determined that the tandem SH2 domain of <i>S. cerevisiae</i> Spt6 binds the linker region of the RNA polymerase II subunit Rpb1 rather than the expected sites in its heptad repeat domain. The 4 nM binding affinity requires phosphorylation at Rpb1 S1493 and either T1471 or Y1473. Crystal structures showed that pT1471 binds the canonical SH2 pY site while pS1493 binds an unanticipated pocket 70 Å distant. Remarkably, the pT1471 phosphate occupies the phosphate-binding site of a canonical pY co ...[more]