Ontology highlight
ABSTRACT:
SUBMITTER: Sun M
PROVIDER: S-EPMC3009887 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Sun Mai M Larivière Laurent L Dengl Stefan S Mayer Andreas A Cramer Patrick P
The Journal of biological chemistry 20101006 53
Spt6 is an essential transcription elongation factor and histone chaperone that binds the C-terminal repeat domain (CTD) of RNA polymerase II. We show here that Spt6 contains a tandem SH2 domain with a novel structure and CTD-binding mode. The tandem SH2 domain binds to a serine 2-phosphorylated CTD peptide in vitro, whereas its N-terminal SH2 subdomain, which we previously characterized, does not. CTD binding requires a positively charged crevice in the C-terminal SH2 subdomain, which lacks the ...[more]