Ontology highlight
ABSTRACT:
SUBMITTER: Pessino V
PROVIDER: S-EPMC5599254 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Pessino Veronica V Citron Y Rose YR Feng Siyu S Huang Bo B
Chembiochem : a European journal of chemical biology 20170622 15
Labeling proteins with high specificity and efficiency is a fundamental prerequisite for microscopic visualization of subcellular protein structures and interactions. Although the comparatively small size of epitope tags makes them less perturbative to fusion proteins, they require the use of large antibodies that often limit probe accessibility and effective resolution. Here we use the covalent SpyTag-SpyCatcher system as an epitope-like tag for fluorescent labeling of intracellular proteins in ...[more]